Paracoccidioides brasiliensis 14-3-3 protein is important for virulence in a murine model

Caroline Maria Marcos; Haroldo César de Oliveira; Patrícia Akemi Assato; Cléverton Roberto de Andrade; Ana Marisa Fusco‐Almeida; Maria José Soares Mendes‐Giannini

doi:10.1093/mmy/myy112

November 15, 2018Medical MycologyOpen Access

Paracoccidioides brasiliensis 14-3-3 protein is important for virulence in a murine model

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Key Points

To assess the contribution of the adhesin Pb14-3-3 to the virulence and pathogenesis of Paracoccidioides brasiliensis during pulmonary infection.
Evaluated a Paracoccidioides brasiliensis strain engineered with downregulated Pb14-3-3 expression via antisense RNA (Pb14-3-3 aRNA) in a murine model of paracoccidioidomycosis.
Analyzed yeast cell morphology and measured the capacity to induce pneumocyte apoptosis in vitro relative to control strains.
Downregulation of Pb14-3-3 significantly attenuated fungal virulence in vivo, resulting in reduced pulmonary fungal burden and fewer granulomas.
Pb14-3-3 aRNA yeast exhibited an altered, elongated cellular morphology.
In vitro assays demonstrated that Pb14-3-3 knockdown markedly impaired the ability of the fungus to trigger pneumocyte apoptosis.

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Implication

Murine experimental study demonstrates reduced fungal burden and granuloma formation in mice, highlighting adhesin Pb14-3-3 as a critical virulence factor.

Abstract

The Paracoccidioides brasiliensis strain downregulated the expression of adhesin Pb14-3-3 (Pb14-3-3 aRNA) was evaluated in a murine model of paracoccidioidomycosis (PCM). Pb14-3-3 aRNA displays attenuated virulence and triggered the formation of fewer granulomas by lowering the fungal burden in the lungs. Additionally, the Pb14-3-3 aRNA showed more elongated yeast cells and less ability to induce pneumocytes apoptosis in vitro. Our results show that 14-3-3 is an important virulence factor in P. brasiliensis-induced pulmonary infection.

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Marcos et al. (2018) studied this question.

synapsesocial.com/papers/6aa9f6dc663efc0f70844241 https://doi.org/10.1093/mmy/myy112

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Paracoccidioides brasiliensis 14-3-3 protein is important for virulence in a murine model

Key Points

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Cite This Study

Paracoccidioides brasiliensis 14-3-3 protein is important for virulence in a murine model

Key Points

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Implication

Abstract

Discussion

Cite This Study

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