Phytochemical Evaluation and HPTLC Fingerprinting for the Standardization of Pachai Pakku Thailam , a Traditional Siddha Herbal Formulation for Non-Healing Ulcers

Objective The Siddha medicinal system is a traditional, regionally established healthcare method that emphasizes both disease prevention and treatment. Pachai Pakku Thailam , a popular herbal oil in this tradition, has been utilized for a long time to treat non-healing ulcers. This research intends to examine the phytochemical constituents and establish an HPTLC fingerprint profile of Pachai Pakku Thailam to facilitate its standardization in Siddha medicine. Methods Pachai Pakku Thailam are analyzed phytochemical screening and HPTLC fingerprint analysis. Silica gel 60 F 254 plates were used for chromatographic separation with toluene: ethyl acetate (93:7, v/v). Stability was evaluated by analyzing chromatographic fingerprints following three and six months of storage. Results Phytochemical compounds, saponins, terpenoids, and steroids, whereas proteins and reducing sugars were not detected. HPTLC analysis yielded a distinct chromatographic fingerprint featuring five prominent peaks at Rf values around 0.05, 0.29, 0.37, 0.60, and 0.89. Similar chromatographic patterns and consistent Rf values were noted over the six-month study duration, demonstrating adequate chemical stability and reproducibility of the formulation. Conclusion The present investigation establishes phytochemical screening and HPTLC fingerprint for Pachai Pakku Thailam , providing scientific evidence for its quality assessment, identity verification, and stability. These findings support its future standardization as a traditional Siddha formulation. Further pharmacological, toxicological, and clinical investigations are required to correlate the chromatographic profile with therapeutic efficacy.