Differential Expression of KCNJ12 Gene and Association Analysis of Its Missense Mutation with Growth Traits in Chinese Cattle

Figures

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  Figure 1. Sequencing result of the g.35989944T>C mutation in the KCNJ12 gene.

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  Figure 2. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of g.35989944T>C by agarose gel electrophoresis.

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  Figure 3. Expression profiling of KCNJ12 in different tissues of three 90-day-old fetuses. The values are the averages of three samples calculated by 2−∆∆Ct. Error bars represent the standard error (SE) (n = 3) calculated by ∆Ct. The GAPDH gene was chosen as the internal reference gene for the qPCR analysis.

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  Figure 4. The effects of g.35989944T>C on KCNJ12 gene expression in 30 muscle tissues. The GAPDH gene was chosen as the internal reference gene for the qPCR analysis. Thirty muscle samples were genotyped, including three TT samples, 15 TC samples, and 12 CC samples.

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  Figure 5. Expression of KCNJ12, MYOD, MYOG, and MYHC genes during myoblast differentiation. Relative mRNA expression levels of KCNJ12, MYOD, MYOG, and MYHC genes in different differentiation periods were analyzed by qPCR in primary bovine skeletal muscle cells. The mRNA expression levels of the four genes were normalized to GAPDH. The values 0–10 have the same unit as 10–200 on the Y-axis. DMD−1: differentiating myoblast day −1; DMD0: differentiating myoblast day 0; DMD1: differentiating myoblast day 1; DMD2: differentiating myoblast day 2; DMD4: differentiating myoblast day 4.