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February 3, 2019Frontiers in EndocrinologyOpen Access

miR-27b-3p inhibition enhances adipose browning, reducing weight gain and improving insulin sensitivity in obese mice.

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Why the study?

Enhancing the browning ability of visceral adipose tissue is of therapeutic interest for central obesity, leading investigators to evaluate the regulatory role of miR-27b-3p in epididymal white adipose tissue browning.

Does inhibition of miR-27b-3p enhance browning of epididymal fat and improve metabolism in HFD-induced obese mice?

Population

High-fat diet induced obese mice

Comparison

Tail vein injection of antimiR-27b-3p vs control

Design

Preclinical animal study

Key result

Inhibition of miR-27b-3p enhanced the browning capacity of epididymal white adipose tissue in high-fat diet-induced obese mice, leading to reduced weight gain and improved insulin sensitivity.

Authors

JYJing YuYLYifan LvFWFengliang Wang

Discussion

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Overview

Supports miR-27b-3p inhibition in obese mice; hypothesis-generating and requires human validation before any clinical consideration.

Structured PICO

Does inhibition of miR-27b-3p enhance browning of epididymal fat and improve metabolism in HFD-induced obese mice?

P
Population
Male C57BL/6J mice fed a high-fat diet for 13 weeks to induce obesity, followed by lentiviral treatment.
I
Intervention
Inhibition of miR-27b-3p via tail vein injection of antimiR-27b-3p
O
Outcome
Browning capacity of epididymal white adipose tissue (eWAT), weight loss, and insulin sensitivitysurrogate

Inhibition of miR-27b-3p enhances browning of epididymal white adipose tissue, leading to weight loss and improved insulin sensitivity in a mouse model of diet-induced obesity.

Limitations

  • Systemic treatment with lentivirus cannot exclude effects of miR-27b-3p inhibition on development and physiology of BAT or skeletal muscle.
  • Skeletal muscle was not assessed in this study.

Cite This Study

Yu et al. (2019) studied High-fat diet induced obesity. antimiR-27b-3p lentivirus vs. scrambled control (scr-miR) lentivirus was evaluated on Browning capacity of epididymal white adipose tissue (eWAT) and insulin sensitivity. Inhibition of miR-27b-3p enhanced the browning capacity of epididymal white adipose tissue in high-fat diet-induced obese mice, leading to reduced weight gain and improved insulin sensitivity.

synapsesocial.com/papers/6aaa24dbf8c08cbefbf6c42chttps://doi.org/10.3389/fendo.2019.00038
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