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Density gradients with increasing concentrations of cyclodextrin and detergent-destabilized liposomes are loaded with detergent-solubilized membrane proteins in a new approach for reconstituting membrane proteins. During centrifugation, the detergent is gradually replaced by the lipid. This gentle method separates proteoliposomes from empty liposomes and is particularly suitable for the lipid reconstitution of large, fragile membrane proteins.
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Althoff et al. (2012) studied this question.
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