Toxicological effects of long-term continuous exposure to ambient air on human bronchial epithelial Calu-3 cells exposed at the air-liquid interface

/24h. For the first time, ALI human lung epithelial cells were exposed for 72 h to continuous airflow of ambient air. Despite direct exposure to ambient aerosols, only small decrease in cell viability and gene expression changes was observed. We propose this experimental set-up combining comprehensive aerosol characterization and long-term continuous ALI cell exposure for the identification of hazardous compounds or compound mixtures in ambient air.