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A method has been developed to detect acetyl-choline-like activity in the perilymph of guinea pigs. The acetylcholine-like activity in the guinea pig perilymph is not significantly increased by moderate acoustical stimuli but it is significantly increased when the crossed olivocochlear bundle is tetanically stimulated. The criteria for identifying acetylcholine as the chemical mediator at the crossed olivo-cochlear nerve/hair cell junction are now largely fulfilled.
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Norris et al. (1974) studied this question.
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