Enhanced prevention on postoperative atrial fibrillation by using anti-inflammatory biodegradable drug patch

Figures

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  Figure 1.. Fabrication of the patch. (A) Schematic illustration of patch fabrication and application. (B) Synthesis of PEL triblock copolymers and characterization of physical properties detection. Fabrication of drug-loaded atrial patch synthesis. 1H-NMR spectrum (C, D) and GPC spectrum (E) of PEL triblock copolymers. (F)The digital photo of the atrial patch. (G, H) Surface cross-sectional and SEM micrographs of the atrial patch. (I) The fluorescence microscopy image of the distribution of RITC-labeled Andr within the patch. (J) The stress–strain curve of the atrial patch. (K) Modulus of the atrial patch at different temperatures (DMA). (L) Concentration standard curve for Andr at a fixed wavelength peak (226 nm). (M) In vitro drug release profiles of Andr-loaded atrial patch. (N) Schematic diagram of the rat model and the patch attached to the left atrium. Retrieve from https://app.biorender.com/biorender-templates.

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  Figure 2.. Andr-loaded patch treatment reduces AF vulnerability in vivo. (A) Diagram of invasive electrophysiological measurement and AF vulnerability detection in vivo. (B) Typical surface ECG (lead II) and intracavitary electrical signal recordings before burst pacing (in sinus rhythm). (C, D) representative simultaneous recordings of surface ECG (lead II) and intracardiac electrograms in rats without or with successfully induced AF. (E) Incidence of pacing-induced AF in rats (n = 6, each rat was measured 5 times). (F) Demonstration of various indicators of electrocardiogram. (G) Quantification of heart rate (n = 6). (H) Quantification of RR interval (n = 6). (I) Quantification of QT interval (n = 6). (J) Quantification of QTc (n = 6). (K) Representative electrical propagation heat map located on the left atrium. Data are shown as mean ± standard deviation (SD) and statistical analysis was performed with one-way ANOVA statistic test. *P < 0.05, **P < 0.01 and ***P < 0.001 indicate statistical significance between paired groups.

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  Figure 3.. Effect of Andr-loaded patch on the atrial repolarization characteristic from the Vm channel. (A) Diagram of the ex vivo optical mapping process. (B) Representative simultaneous recordings located on the left atrial epicardium without or with success fully induced AF. (C) The inducibility of atrial arrhythmia induced by burst pacing of four groups; *P < 0.05 indicates statistical significance between paired groups (n = 6, each rat heart was measured 5 times). (D) Quantitative statistics of atrial conduction velocities among different S1–S1 intervals from 240 ms to 160 ms, *P < 0.05 vs. Sham group; #P < 0.05 vs. blank-loaded group; &P < 0.05 vs. Andr-loaded group. (E) Representative heatmaps and trajectories of APD80 among four groups, *P < 0.05 vs. Sham group (n = 6); #P < 0.05 vs. blank-loaded group (n = 6); &P < 0.05 vs. Andr-loaded group (n = 6); representative time to peak maps and typical action time heatmaps on AP channel of four groups; quantitative analysis of APD differences from APD20 to APD90 and its COV, *P < 0.05 vs. control group (n = 6); &P < 0.05 vs. Andr-loaded group (n = 6); statistic analysis of peak maps on AP channel. *P < 0.05 indicates statistical…

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  Figure 4.. Andr-loaded patch treatment attenuated atrial inflammation in vivo and in vitro. (A) Representative images of hematoxylin-eosin (HE) staining. (B) Representative images of M1 macrophage polarization in the atrial of each group. M1 macrophage was labeled by inos positive together with CD68 positive. (C) Quantitative analysis of the proportion of M1 macrophage (n = 3). (D) Quantitative analysis of the expression of TNF-α and IL-1β (n = 3). (E) Diagram of the isolation and simulation of the BMDMs. (F) Representative images of propidium iodide (PI)-positive apoptotic BMDMs determined by flow cytometry. Quantitative analysis of the PI-positive apoptotic BMDMs (n = 3). (G) Representative images of the CD86-positive BMDMs determined by flow cytometry. Quantitative analysis of the CD86-positive BMDMs (n = 3). (H) TNF-α levels in BMDMs medium determined by ELISA (n = 3). (I) Representative images of ROS level in BMDMs using DCFH-DA probe (n = 3). Quantitative analysis of the DCF fluorescence density. Data are shown as mean ± standard deviation (SD), and statistical analysis was performed with one-way ANOVA statistic test. *P < 0.05, **P < 0.01 and ***P < 0.001 indicate statistical…

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  Figure 5.. Andr-loaded patch treatment alleviated oxidative stress injury in vivo and in vitro. (A) Representative dihydroethidium (DHE) fluorescence images of superoxide production in rats. (B) Quantitative analysis of the fluorescence density of DHE (n = 3). (C) Representative blots of heme oxygenase-1 (HMOX1), NQO1, SOD1 and SOD2. (D) Quantitative analysis of the expression of HMOX1, NQO1 and SOD2 (n = 3). (E) qPCR was carried out to determine the mRNA levels of HMOX1, NQO1 (data were normalized to GAPDH, n = 3). (F) Representative images of DCFH-DA HL-1 cell determined by flow cytometry. (G) Representative Fluo4 fluorescence images of Ca2+ and JC-1 mitochondrial membrane potential (MMP) assay in HL-1 cells. (H) Quantitative analysis of the fluorescence density of DCF, Fluo4 and JC-1 (n = 3). Data are shown as mean ± standard deviation (SD), and statistical analysis was performed with one-way ANOVA statistic test. *P < 0.05, **P < 0.01 and ***P < 0.001 indicate statistical significance between paired groups.

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  Figure 6.. Effect of Andr-loaded patch on the atrial calcium handling capabilities, the arrhythmogenic alternans and re-entry ex vivo. (A) Representative heatmaps and trajectories of CaD80 among four groups, *P < 0.05 vs. Sham group (n = 6); #P < 0.05 vs. blank-loaded group (n = 6); &P < 0.05 vs. Andr-loaded group (n = 6); representative time to peak maps and typical action time heatmaps on Ca2+ channel of four groups; quantitative analysis of CaD differences from CaD20 to CaD90 and its COV, *P < 0.05 vs. control group (n = 6); &P < 0.05 vs. Andr-loaded group (n = 6); statistic analysis of peak maps on Ca2+ channel. *P < 0.05 indicates statistical significance between paired groups (n = 6). (B) Representative Ca2+ alternans among four groups at different S1S1 pacing intervals; quantitative calculations of Ca2+ alternans ratio among four groups, *P < 0.05 vs. Sham group (n = 6); &P < 0.05 vs. Andr-loaded group (n = 6); (C) Representative schematic diagrams of S2 induction (showed in the dashed box) after five consecutive S1 stimuluses in four groups; calculation of the recovery ratio of CaT ratio among four groups and its statistical analysis for the COV-recovery of CaT ratio, *P < 0.05 vs.…

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  Figure 7.. Therapeutic effect of Andr-patch on POAF by regulating the ROS balance and Ca2+. Retrieve from https://app.biorender.com/biorender-templates.