Endogenous generation and protective effects of nitro-fatty acids in a murine model of focal cardiac ischaemia and reperfusion
Key result
Nitro-oleic acid during ischemia cuts infarct size ~46% and preserves LV function in mice.
- 46% reduction
Why the study?
The in vivo generation and therapeutic effects of nitrated fatty acids in myocardial ischaemia and reperfusion injury were not fully characterized.
Does exogenous administration of nitro-oleic acid reduce infarct size and preserve left ventricular function in a murine model of myocardial ischaemia and reperfusion?
Population
C57/BL6 mice subjected to 30 min coronary artery ligation and reperfusion
Comparison
Exogenous OA-NO2 administration vs vehicle treatment
Design
Preclinical in vivo murine model study
Follow-up
30 min reperfusion
Authors
Discussion
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Member takes
Implication
Warrants larger-animal validation; leaves open translation to human myocardial ischemia therapy.
Structured PICO
Does exogenous administration of nitro-oleic acid reduce infarct size and preserve left ventricular function in a murine model of myocardial ischaemia and reperfusion?
P
Population
C57/BL6 mice subjected to 30 min of coronary artery ligation to model myocardial ischaemia and reperfusion injury.
I
Intervention
Exogenous administration of 20 nmol/g body weight nitro-oleic acid (OA-NO2) during the ischaemic episode
C
Comparator
Vehicle-treated mice
O
Outcome
Infarct size (normalized to area at risk) and left ventricular functionsurrogate
Main Result
Effect estimate: 46% reduction
Exogenous administration of nitro-oleic acid protects against myocardial ischaemia/reperfusion injury in mice by reducing infarct size and preserving left ventricular function, partly via NFkappaB inhibition.
Cite This Study
Rudolph et al. (2009) studied myocardial ischaemia and reperfusion injury. nitro-oleic acid (OA-NO(2)) vs. vehicle was evaluated on infarct size (normalized to area at risk) (46% reduction). Exogenous administration of nitro-oleic acid during ischaemia reduced infarct size by 46% and preserved left ventricular function compared with vehicle-treated mice.