Description of a new Gill titration calorimeter for the study of biochemical reactions. II: operational characterization of the instrument

For part I see El Harrous et. al., ibid., vol.5, pp.1065-70 (1994). This paper continues the description of an isothermal titration calorimeter already outlined in the previous paper. We have determined its thermal parameters and report here on the performance of the calorimeter and the method of measuring via electrical compensation of the thermal effect. We have also calculated the effective volume of the reaction vessel and the heat of stirring of the reaction mixture in the vessel, and of injecting water into water. Finally, correct working of the calorimeter has been tested by a dilution experiment of a saccharose solution.