Isolates of Leptosphaerulina spp. from alfalfa (Medicago sativa) and white clover (Trifolium repens) were compared for growth rate on V-8 juice medium at 12-28 C, colony characteristics, production of pseudothecia, ascospore morphology, and pathogenicity on alfalfa and white clover. Optimum growth rate occurred at 20-24 C. Isolates from white clover generally had slower, restricted growth with irregular colony margins on agar medium, whereas isolates from alfalfa generally grew rapidly with regular colony margins (...)
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A 1990 study studied this question.