Key result
Bub1 insufficiency prevents renal failure and improves survival in septic mice by preserving endothelial anticoagulation.
Why the study?
The role of Bub1 in controlling vascular endothelial function and its impact on acute renal failure in severe sepsis was unclear.
Does Bub1 insufficiency prevent acute renal failure and death in severe sepsis?
Does Bub1 insufficiency prevent acute renal failure and death in severe sepsis?
Bub1 insufficiency prevents acute renal failure and improves survival in severe sepsis by maintaining anticoagulant functions of vascular endothelial cells via Akt and JNK signaling.
Caution against clinical use of Bub1 targeting in sepsis; leaves open its potential to preserve endothelial anticoagulation in human studies.
Severe sepsis is critical to health and can result in acute renal failure (ARF). Tissue factor (TF) and thrombomodulin (TM) play key roles in vascular endothelial functions by helping maintain microcirculation in the kidney. Budding uninhibited by benzimidazole-1 (Bub1) plays a role in Akt and JNK signaling, which control TF and TM, respectively. We hypothesized that Bub1 could control vascular endothelial function in sepsis. The aim of this study was to determine the role of Bub1 in septic ARF. We used Mouse cecum ligation and puncture (CLP) using low Bub1 expressing (Bub1) and wild-type (Bub1) mice in vivo and lipopolysaccharide (LPS) stimulation of human aortic endothelial cell (HAEC) in vitro. Bub1 mice had a higher survival rate after CLP than Bub1. Bub1 mice had more severe ARF after CLP than Bub1 with blood biochemical and pathological analyses. TF expression in Bub1 mice and control HAEC (control) significantly increased in the septic model compared with Bub1 and Bub1 silenced HAEC (siBub1). TM expression in the control significantly decreased after LPS stimulation compared with siBub1. Akt and JNK phosphorylation of siBub1 were attenuated after LPS stimulation. Associations of Bub1 with Akt or JNK after LPS stimulation of HAEC were detected using immunoprecipitation, suggesting that Bub1 is involved in the phosphorylation of Akt and JNK after LPS stimulation. Bub1 insufficiency attenuates TF expression and reduces TM suppression by blocking Akt and JNK phosphorylation, respectively, thus leading to the prevention of ARF and death caused by sepsis.
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Matsubara et al. (2018) studied Severe sepsis and acute renal failure. Bub1 insufficiency (low Bub1 expression / silencing) vs. Wild-type (Bub1) mice and control human aortic endothelial cells was evaluated on Survival rate and severity of acute renal failure. Bub1 insufficiency prevented acute renal failure and improved survival in a mouse model of severe sepsis by maintaining anticoagulant functions of vascular endothelial cells.
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