Efficacy evaluation of enzyme-treated Nypa fruticans extracts

Nypa fruticans has long served various purposes in daily life. Recent studies have explored the physiological properties, such as antioxidant and anti-inflammatory activities of N. fruticans secondary metabolites, along with methods to improve extraction efficiency. This study evaluated the efficacy of an enzyme-treated N. fruticans extract, as well as its extraction efficiency after hydrolytic enzyme treatment. Based on bioactive compound analysis, the optimal treatment temperature for N. fruticans's cellulase and viscozyme was confirmed to be 55°C. The polyphenol and flavonoid contents of enzyme-treated N. fruticans, extracted using 50% ethanol, were determined to be 179.8 and 102.6 mg/g, respectively. The DPPH radical scavenging ability and SOD-like activity of the enzyme-treated N. fruticans extract were determined to be 74.9% and 19.8%, respectively, at 200 mg/L. No cytotoxicity was observed below 100 μg/mL. At this concentration, the extract inhibited NO production by 52.4%. The enzyme-treated N. fruticans extract exhibited various physiological activities, highlighting its potential as a functional food ingredient.