Key result
New HPLC method with fluorescence detection successfully quantifies carvedilol in human plasma.
Why the study?
A simple, rapid, and sensitive method to quantify carvedilol in human plasma was needed for clinical and pharmacokinetic studies.
A simple, rapid, and sensitive HPLC method with fluorescence detection was successfully developed and validated for quantifying carvedilol in human plasma.
May enable carvedilol plasma monitoring in hypertension; leaves open routine use pending larger validation studies.
A simple, rapid and sensitive high-performance liquid chromatography (HPLC) method has been developed to quantify carvedilol in human plasma using an isocratic system with fluorescence detection. The method included a single-step liquid-liquid extraction with diethylether and ethylacetate mixture (3 : 1, v/v). HPLC separation was carried out by reversed-phase chromatography with a mobile phase composed of 20 mM phosphate buffer (pH 7)-acetonitrile (65 : 35, v/v), pumped at a flow rate of 1.0 mL/min. Fluorescence detection was performed at 240 nm (excitation) and 330 nm (emission). The calibration curve for carvedilol was linear from 10 to 250 ng/mL. Intra- and interday precision values for carvedilol in human plasma were <4.93%, and accuracy (relative error) was better than 4.71%. The analytical recovery of carvedilol from human plasma averaged out to 91.8%. The limits of detection and quantification of carvedilol were 3.0 and 10 ng/mL, respectively. Also, the method was successfully applied to three patients with hypertension who had been given an oral tablet of 25 mg carvedilol.
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Yılmaz et al. (2015) studied Hypertension (n=3). Carvedilol was evaluated on Quantification of carvedilol in human plasma. A newly developed HPLC method with fluorescence detection successfully quantified carvedilol in human plasma, demonstrating linearity from 10 to 250 ng/mL and application in 3 hypertensive patients.
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