Endo-polygalacturonase II from Coniothyrium diplodiella has been purified. The purified enzyme was homogeneous on ultracentrifugation and free-boundary electrophoresis. Its sedimentation coefficient was approximately 3.3S. The enzyme was stable at 45°C and in the pH range of 4.0_??_6.0, but completely inactivated by heating at 60°C for 10 minutes. The enzyme carried out a random hydrolysis of pectic acid resulting in a mixture of digalacturonic and galacturonic acids as the end products of hydrolysis. The extent of hydrolysis of pectic acid was 68.8 per cent. The ratio of VRu (viscosity-reducing activity) to PGu (reducing group-releasing activity) was 1.32.
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Akira ENDO (1964) studied this question.