We obtained four monoclonal antibodies to Pythium ultimum using a crude preparation of P. ultimum cell wall material as the immunogen. Initial selection was based on positive reactions with a culture of P, ultimum and negative reactions with one isolate each of P. irregulare and Phytophthora cinnamomi in indirect enzyme-linked immunosorbent assay (ELISA). The antibodies were screened further against 21 isolates of P, ultimum (including 11 asexual strains), 39 isolates of 16 other Pythium spp., and 24 isolates of 12 other genera of soil fungi. Antibody ES, the most specific to P. ultimum, was highly reactive to all P. ultimum isolates and had low or no reactivity to other Pythium spp
No takes yet. Share an insight, caveat, or question.
Gary Y. Yuen (1993) studied this question.