Key result
Generating expressed sequence tags from Glycyrrhiza uralensis yields ~10,000 unique sequences to understand glycyrrhizin biosynthesis.
Why the study?
Glycyrrhiza uralensis is an important medicinal plant, but detailed gene expression data from its rhizomes were lacking.
Answers are grounded in this paper and cite their sources.
This study provides a large dataset of expressed sequence tags for Glycyrrhiza uralensis, facilitating future genetic and functional studies of this medicinal plant.
Glycyrrhiza uralensis (Leguminosae, Fabaceae) is the most important medicinal plant used as a natural sweetener, in Chinese herbal medicines, and as a tobacco flavoring agent. Here, we report the generation of 56,857 expressed sequence tags (ESTs) derived from two full-length cDNA libraries produced from rhizomes (stolons) of two strains of G. uralensis, together with differential expression data obtained by MegasortTM analysis. The MegasortTM analysis was performed using two plant organs, namely, thickened roots and a rhizome-like organ, which differed in their glycyrrhizin content. After clustering of the 5′-end of ESTs, 5,542 contigs and 4,932 singletons were generated. A total of 10,474 nonredundant sequences were annotated on the basis of the TAIR database, of which 7,905 (75.5%) sequences exhibited homology to the sequences of registered genes. The gene functions of these 7,905 sequences were classified into Biological Process and Molecular Function on the basis of Gene Ontology (GO).
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Sudo et al. (2009) studied this question. Researchers generated 56,857 expressed sequence tags from rhizomes of Glycyrrhiza uralensis, identifying 10,474 unique sequences to aid in understanding the biosynthesis of glycyrrhizin.
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