Key result
Human embryonic myosin heavy chain cDNA maps to chromosome 17 with ~97% rat sequence homology.
Why the study?
The sequence information of human embryonic myosin heavy chain was needed to study gene structure and its role in normal and defective human myogenesis.
Key points are not available for this paper at this time.
Population
Human fetal skeletal muscle tissue
Design
Isolation and characterization of cDNA clone from expression library
No immediate clinical application; extends molecular tools for studying cardiac myosin expression.
A 3.6 kilobase cDNA clone coding for the human embryonic myosin heavy chain has been isolated and characterized from an expression library prepared from human fetal skeletal muscle. The derived amino acid sequence for the entire rod part of myosin shows 97% sequence homology between human and rat and a striking interspecies sequence conservation among the charged amino acid residues. The single copy gene is localized to human chromosome 17 and its expression in fetal skeletal muscle is developmentally regulated. The sequence information permits the design of isoform-specific probes for studies on the structure of the gene and its role in normal and defective human myogenesis.
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Eller et al. (1989) studied this question. A 3.6 kilobase cDNA clone coding for the human embryonic myosin heavy chain was isolated, showing 97% sequence homology with rat and localization to human chromosome 17.
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