Functional analysis of differentially expressed circular RNAs in sheep subcutaneous fat

Figures

• 

  Fig. 1. General Characteristics of circRNAs in the Sheep A Number of circRNAs identified in Duolang sheep (D Group) and Small Tail Han sheep (X Group). B Distribution of circRNAs in fat of sheep. The X axis represents the length distribution of circRNAs, and the Y axis represents the number of circRNAs. C Distribution of the number of circRNA exons. The X axis represents the number of exons, and the Y axis represents the number of circRNAs. D Classification of circRNA in subcutaneous fat tissue of sheep

• 

  Fig. 2. The volcano. The X-axis represents Log2(Fold Change); The Y-axis represents -log10 (P-value); Red dots indicate significant up-regulation, blue dots indicate significant down-regulation, and gray indicates no significant difference

• 

  Fig. 3. Regulatory networks of genes. CircRNA-miRNA regulatory network in sheep subcutaneous fat tissue. The circle represents circRNA, and the arrow represents miRNA, The red represents up-regulated expression in Duolang sheep, and the green represents down-regulated expression. Node size indicates degree

• 

  Fig. 4. A GO enrichment analysis and B KEGG pathway analysis of circRNA source genes. C enrichment analysis and D KEGG pathway analysis of the target genes of circRNAs

• 

  Fig. 5. Target mRNAs of subcutaneous fat tissue associated with circRNAs. A Each column represents a group, and each row represents a transcription. The red represents a higher expression level of the transcription in the group, and blue represents a lower expression level. The specific expression size trend is shown in the upper left color bar. On the left is the transcription cluster tree. The closer the branches are to each other, the transcript name is in the middle, and the corresponding gene name is on the right side. B Subcellular localization of all target genes. C Prediction of protein motif localization

• 

  Fig. 6. circRNA-miRNA-mRNA interaction networks. Red circles represent up-regulated circRNAs in Duolang sheep subcutaneous fat tissue. Green arrows represent down-regulated miRNA in Duolang sheep subcutaneous fat tissue. Red squares represent up-regulated mRNA in Duolang sheep subcutaneous fat tissue

• 

  Fig. 7. Validation of RNA-Sequencing (RNA-Seq) data using qRT-PCR. A qRT-PCR, B RNA-seq.The qRT-PCR validation of differentially expressed circRNAs, miRNAs and mRNAs. The abscissa is differentially expressed RNAs, the ordinate is relative expression. The dark is group D, light is group X. Note: *: P < 0.05; **: P < 0.01; ***: P < 0.001