Protein Binding Study of Perillyl Alcohol Enantiomers by High Performance Frontal Analysis

High‐performance frontal analysis (HPFA) was used for protein binding study of perillyl alcohol (POH) enantiomers to human serum albumin (HSA). The analysis was performed on a Develosil 100‐Diol‐5 (10 cm × 4.6 mm I.D.) column. Sodium phosphate solution (pH 7.4, ionic strength 0.17) was used as the mobile phase at a flow rate of 1 mL/min. UV wavelength was set at 205 nm. An injection volume of 600 µL was chosen to ensure the drug to be eluted as a trapezoidal peak with a plateau. Experimental data were fitted by Scatchard analysis. The binding constant (K) and binding affinity (nK) of POH enantiomers to HSA were: K = 5.20 × 106 (1/mol), nK = 1.74 × 104 (1/mol) for (S)‐POH and K = 3.71 × 106 (1/mol), nK = 1.51 × 104 (1/mol) for (R)‐POH, respectively.