Key result
Severe obesity linked to ~31% lower lipid accumulation in differentiating subcutaneous ASCs versus normal-weight controls.
Why the study?
Impaired adipogenesis leading to dysfunctional adipocytes contributes to obesity-associated metabolic syndrome, but its relationship with metabolic and hormonal profiles in bariatric surgery candidates was unclear.
Case-Control (n=27)
No
In severely obese females, abnormal adipogenesis is associated with insulin resistance and altered adipokine profiles, which may predict future type 2 diabetes development.
BACKGROUND: The adipocyte expansion is a critical process with implications in the pathogenesis of obesity associated metabolic syndrome. Impaired adipogenesis leads to dysfunctional, hypertrophic adipocytes, local inflammation and peripheric insulin resistance. METHODS: We assessed the relationship between the adipogenic differentiation capacity of the subcutaneous adipose derived stem cells (ASCs), evaluated by total lipid accumulation, and the metabolic and hormonal profile in a group of obese female patients proposed for bariatric surgery (N = 20) versus normal weight female controls (N = 7). RESULTS: The lipid accumulation (measured as optical density at 492 nm) of ASCs during their differentiation to adipocytes was significantly lower in ASCs isolated from obese patients as compared to ASCs isolated from normal weight patients (0.49 ± 0.1 vs. 0.71 ± 0.1, p < 0.001). Significant negative correlations between lipid accumulation in adipogenic differentiated ASCs and plasma concentrations of triglycerides (p < 0.01), insulin (p < 0.001), HOMA-IR (p < 0.01), adiponectin (p < 0.05) and leptin/adiponectin ratio (p < 0.05) were found in obese group. CONCLUSIONS: In severely obese female patients, the abnormal adipogenesis is related to insulin resistance and leptin/adiponectin ratio. The abnormal lipid accumulation in the mature adipocyte derived from obese ASCs could possible predict the further development of type 2 diabetes mellitus in severely obese patients and influence the selection of patients for bariatric surgery.
No takes yet. Share an insight, caveat, or question.
Hristov et al. (2019) conducted a case-control in Obesity (n=27). Severe obesity vs. Normal weight was evaluated on Lipid accumulation (optical density at 492 nm) of ASCs during differentiation to adipocytes (p=<0.001). Severe obesity was associated with significantly lower lipid accumulation in differentiating subcutaneous adipose-derived stem cells compared to normal-weight controls (0.49 vs 0.71 OD, p<0.001).
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: