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Develop a multifunctional deep eutectic solvent system that prevents lignin condensation and protects cellulase enzymes to achieve one-pot biomass fractionation and in situ saccharification without intermediate washing steps.

September 17, 2026ACS Sustainable Chemistry & Engineering

Synergistic Carbocation Quenching and Bioprotective Shielding for Uncondensed Lignin and In Situ Saccharification

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Authors

ZQZhao Qin IRIqra Rustam JZJikai Zong

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Overview

Experimental study demonstrates efficient biomass fractionation and enzymatic saccharification using a multifunctional deep eutectic solvent, indicating enhanced biorefinery sustainability.

Key Points

Develop a multifunctional deep eutectic solvent system that prevents lignin condensation and protects cellulase enzymes to achieve one-pot biomass fractionation and in situ saccharification without intermediate washing steps.
Guided solvent selection using Conductor-like Screening Model for Real Solvents (COSMO-RS) thermodynamic calculations to formulate a deep eutectic solvent composed of choline prolinate ([Ch][Pro]) and gallic acid (GA).
Conducted one-pot biomass delignification followed directly by in situ enzymatic hydrolysis (cellulase at 50 °C for 24 h) without intermediate biomass washing.
Evaluated solvent recyclability across five cycles using a 150 Da nanofiltration and diafiltration process to remove accumulated lignin fragments, furans, and dimers.
The [Ch][Pro]/GA solvent system preserved 91.0% uncondensed lignin with 82.3% intact β–O–4 linkages and an 8.4% condensation rate through nucleophilic carbocation quenching.
Gallic acid formed a bioprotective shield retaining 98.2% of native cellulase α-helical structure, 92.9% relative enzyme activity, and over 95% cellulose preservation, producing a 90.2% total reducing sugar yield after 24 hours.
Nanofiltration regeneration achieved 94.8% removal of lignin fragments and 91.2% removal of furfural/HMF, maintaining 93.5% delignification and 91.8% enzyme compatibility after five cycles.

Cite This Study

Qin et al. (2026) studied this question.

synapsesocial.com/papers/6aabb6c75f706d05830e573a https://doi.org/10.1021/acssuschemeng.6c08548

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September 17, 2026ACS Sustainable Chemistry & Engineering

Synergistic Carbocation Quenching and Bioprotective Shielding for Uncondensed Lignin and In Situ Saccharification

View Full Paper

Ask AI

Bookmark

Authors

ZQZhao Qin IRIqra Rustam JZJikai Zong

Discussion

Member takes

Overview

Experimental study demonstrates efficient biomass fractionation and enzymatic saccharification using a multifunctional deep eutectic solvent, indicating enhanced biorefinery sustainability.

Key Points

Develop a multifunctional deep eutectic solvent system that prevents lignin condensation and protects cellulase enzymes to achieve one-pot biomass fractionation and in situ saccharification without intermediate washing steps.
Guided solvent selection using Conductor-like Screening Model for Real Solvents (COSMO-RS) thermodynamic calculations to formulate a deep eutectic solvent composed of choline prolinate ([Ch][Pro]) and gallic acid (GA).
Conducted one-pot biomass delignification followed directly by in situ enzymatic hydrolysis (cellulase at 50 °C for 24 h) without intermediate biomass washing.
Evaluated solvent recyclability across five cycles using a 150 Da nanofiltration and diafiltration process to remove accumulated lignin fragments, furans, and dimers.
The [Ch][Pro]/GA solvent system preserved 91.0% uncondensed lignin with 82.3% intact β–O–4 linkages and an 8.4% condensation rate through nucleophilic carbocation quenching.
Gallic acid formed a bioprotective shield retaining 98.2% of native cellulase α-helical structure, 92.9% relative enzyme activity, and over 95% cellulose preservation, producing a 90.2% total reducing sugar yield after 24 hours.
Nanofiltration regeneration achieved 94.8% removal of lignin fragments and 91.2% removal of furfural/HMF, maintaining 93.5% delignification and 91.8% enzyme compatibility after five cycles.

Cite This Study

Qin et al. (2026) studied this question.

synapsesocial.com/papers/6aabb6c75f706d05830e573a https://doi.org/10.1021/acssuschemeng.6c08548

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