The stimulating effect of short peptides on cellular proliferation in organotypic tissue culture

Proliferation and apoptosis present an important research topic in modern physiology and medicine. Saint Petersburg Institute of Bioregulation and Gerontology developed the technology of extracting polypeptide complexes from different organs and tissues of the calf. These polypeptides stimulate cellular proliferation in culture of different tissues. A polypeptide complex contains short peptides that can possess the same biological activity. The goal of this investigation was to discover the effect of short peptides on cellular proliferation in organotypic culture of brain cortex, liver and spleen tissues of young (3 months) and old (18 months) rats. It was established that effective concentrations of peptides Glu-Asp-Arg, Glu-Asp-Pro, Glu-Asp-Leu and Asp-Ser, Asp-Leu, Asp-Ala, Asp-Gly, Asp-Arg, Ala-Gly stimulate statistically significant (p < 0.05 as compared with controls) cellular proliferation in explants of brain cortex, liver and spleen tissues of young and old rats. The obtained data create the basis for the development of new medicinal drugs, including geroprotectors.