Key result
Baiying Qingmai Formulation ameliorates thromboangiitis obliterans in rats by inhibiting HMGB1/RAGE/NF-κB and MAPK pathways.
Why the study?
Baiying Qingmai Formulation effectively relieves symptoms of thromboangiitis obliterans, but its anti-TAO mechanisms remain unclear.
Does Baiying Qingmai Formulation ameliorate thromboangiitis obliterans by inhibiting HMGB1/RAGE/NF-κB signaling pathways in a rat model?
Does Baiying Qingmai Formulation ameliorate thromboangiitis obliterans by inhibiting HMGB1/RAGE/NF-κB signaling pathways in a rat model?
Answers are grounded in this paper and cite their sources.
Baiying Qingmai Formulation ameliorates thromboangiitis obliterans in rats by inhibiting the HMGB1/RAGE/NF-κB signaling pathways, providing a potential mechanism for its clinical efficacy.
Baiying Qingmai Formulation (BF) is a classical clinical prescription used for decades to treat thromboangiitis obliterans (TAO). Although it effectively relieves pain and ischemic ulcers in patients with TAO, its anti-TAO mechanisms remain unclear. The chemical components of BF were analyzed using high-performance liquid chromatography and the potential targets of the compounds identified in BF were analyzed using molecular docking. Further, the signaling pathways and molecular mechanism of BF in treating TAO were studied using a rat model of TAO. Seven compounds (gallic acid, catechin, chlorogenic acid, caffeic acid, paeoniflorin, quercetin, and paeonol) were identified in BF, and molecular docking predicted their high affinities with HMGB1/RAGE/NF-κB proteins. In in vivo studies, BF not only inhibited the protein expression of HMGB1, RAGE, ICAM-1, and VCAM-1; mRNA levels of HMGB1 and RAGE; and the phosphorylation of NF-κB, ERK, Janus kinase (JNK) and p38 MAPK in the femoral artery, but also reduced the levels of inflammatory cytokines (IL-6, TNF-α, IL-1β, HMGB1) and stable metabolite (TXB2) of cytokine promoting thrombosis (TXA2) in the plasma. Moreover, BF stimulated the secretion of stable metabolite (6-keto-PGF1α) of cytokine inhibiting thrombosis (PGI2) in the plasma. BF inhibited the inflammatory response and thrombosis in the femoral artery, thus reducing the degree of vascular occlusion, which alleviated the symptoms in rats with TAO. Our findings suggest that BF ameliorates TAO by inhibiting the activation of the ERK, JNK, p38 MAPK and HMGB1/RAGE/NF-κB signaling pathways, thereby providing novel ideas for the treatment of TAO and essential information for the further development and utilization of BF as a promising drug to treat TAO.
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Zou et al. (2022) studied Thromboangiitis obliterans (n=60). Baiying Qingmai Formulation vs. Vehicle (0.5% CMC-Na) and Cilostazol was evaluated on Vascular occlusion and inflammatory cytokine levels (IL-6, TNF-α, IL-1β, HMGB1) (p=<0.01). Baiying Qingmai Formulation ameliorated thromboangiitis obliterans in a rat model by inhibiting HMGB1/RAGE/NF-κB and MAPK signaling pathways, significantly reducing inflammatory cytokines and vascular occlusion.
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