We demonstrate that by using constant wavelength synchronous fluorescence spectrometry (CW-SFS) critical concentrations of some types of aggregation can be quantified. Detection of aggregation associated with hydrogen bonding in Chlorin e6 and Triton X-100 suggest that CW-SFS may be a technique that can explore aggregation at much lower levels (dimer, trimer, oligomer, etc.)
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Yu et al. (2011) studied this question.
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