A new dechorionatiog solution for goldfish eggs was developed. A physiological saline solu-tion for freshwater fish containing 0.1% trypsin and 0.4% urea and adjusted to pH 7 was found perfect for dechorionation. Eggs were denuded completely within ten minutes in the solution and some of them developed to the hatching stage in the sterile Ringer's solution. Elastodisk connec-tion was sometimes observed at the 16-32 cell stage.
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Yamaha et al. (1986) studied this question.