Control of microbial contamination in dental unit water systems using tetra-sodium EDTA

AIM: To examine the efficacy of tetra-sodium EDTA in controlling microbial contamination of dental unit water systems (DUWS). METHODS AND RESULTS: Ten dental units were treated once a week with either 4% or 8% tetra-sodium EDTA for four or two consecutive weeks, respectively. Before treatment, 43% and 60% of the water samples from the air/water triple syringe and high-speed hand-pieces, respectively, exceeded the American Dental Association (ADA) guidelines of 200 CFU ml(-1) water during a 6-week baseline period. After each weekend treatment, the levels of microbial contamination in all DUWS fell significantly (P < 0.001) to below the ADA guideline. By the end of the week, microbial counts in the outflowing water had returned to baseline levels indicating a transient effect of single doses of tetra-sodium EDTA, and the need for multiple applications. The biofilms were virtually eliminated after a single weekend treatment. CONCLUSIONS: Tetra-sodium EDTA is effective in controlling microbial contamination in DUWS. SIGNIFICANCE AND IMPACT OF THE STUDY: Inexpensive, effective and safe products for reducing the microbial load of water from DUWS are needed to meet ADA and other national guidelines. Tetra-sodium EDTA can significantly reduce microbial biofilms and bacterial counts in outflowing water, and is compatible for use in DUWS.