The production of interferon by different human cell lines after stimulation by viruses and by poly-I:C was compared. It was found that under optimal conditions, yields of interferon obtained after stimulation with poly-I:C were higher than those obtained after induction by viruses. The production of interferon in poly-I: C-stimulated skin fibroblasts could be increased by pre-treatment with small amounts of poly-I:C (priming) or by inclusion of cycloheximide in the medium. Omission of serum from the medium resulted in a threefold decrease of interferon production. The interferon yield per cell and the specific activity of the interferon prepared in serum-free medium compared favorably with values reported in the literature for human leukocyte interferon.
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Billiau et al. (1972) studied this question.