The objective of this immunohistochemical study was to detail the distribution patterns of α and β integrin subunit expression in non-ischemic non-human primate brain in comparison to that reported for rodents and humans. For this purpose cerebral specimens were obtained from 3 adolescent male baboons by direct perfusion and immediate processing. Well-characterized monoclonal and polyclonal antibodies against human α and β integrin subunit antigens were used to define their location and distribution relative to the markers for vascular basal lamina, laminin (LM), and astrocytic fibers or glial fibrillic acid protein (GFAP). Quantitation of microvascular epitopes required computerized videoimaging microscopy and laser confocal microscopy (LCM). Each subunit was categorized into one of four microvascular patterns relative to LM antigen : (a) all microvascular diameter categories-α 1 , α 6 , and β 1 ; (b) antigen-sparing capillaries α 2 , α 4 , α 5 , α v , and β 3 ; (c) a subset of all microvascular diameter classes-α 3 , β 4 , and β 5 ; and (d) no antigen apparent, β 2 . Subunit antigens α 1 , α 2 , β 1 , and β 5 were detected on perivascular astrocytes. Subunits α 1 and β 1 , and GFAP colocalized (LCM). This quantitative survey detailed specific microvascular and astrocyte associations of certain α and β integrin subunits in non-human primate brain. Specific reproducible and consistent distributions of α and β subunits form the basis for further investigation of their responses to focal ischemia in a human relevant system.
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Haring et al. (1996) studied this question.