Rainbow trout (Salmo gairdneri) kept in a tank with tap water supply for 18 months at a water temperature of 8–13 C were given monthly intraperitoneal injections of nonpathogenic cell-culture-derived Egtved virus. After 1 year, a low titer of neutralizing antibodies to Egtved virus could be demonstrated in the trout serum. Such antibodies could not be demonstrated by the method applied in sera from rainbow trout exposed to natural infection with Egtved virus at two Danish trout farms.
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P. E. Vestergård Jørgensen (1971) studied this question.
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