Preparation of nanoliposomes containing Rosmarinus offi cinalis L essential oil; A comparative study

According to progressive trend of herbal application in modern medicine, various studies has been shown several properties for Rosemary essential oil (EO) such as antioxidant, anti-bacterial, anti-cancer and anti-infl ammatory effect.Owing to liposomal benefi ts such as increased solubility, enhanced performance and increased stability of its content, the main objective of this study was to design nanoliposomes containing rosemary essential oils to achieve more effi cacy.Nano-liposomes containing EO was prepared by three different methods including thin fi lm hydration, sonication and extrusion methods.The physical properties of nanoliposomes such as particle size, poly dispersity index, zeta potential, encapsulation effi ciency and release profi le of EO were studied.The mean size of liposomes containing essential oils of rosemary prepared by sonication method was 162 nm which, is greater than extruder method (470 nm) and liposomes were prepared by thin fi lm hydration were about one μm.Encapsulation effi ciency was higher in sonication method rather that extrusion method.Both methods lead to spherical particles.Release of EO from liposomes in aqueous media was negligible (P value > 0.05).It was found that the method of liposomes preparation, cholesterol concentration and essential oil concentration is effective on the size and encapsulation effi ciency of nanoliposome.