Virus infections of marine fish erythrocytes: electron microscopical studies on the blenny virus

This paper reports for the first time the application of the direct negative staining technique for the rapid confirmation of the viral nature of blenny‘piscine erythrocytic necrosis (PEN). The virus in negatively stained preparations has an internal core structure which appears in exact opposite contrast to that seen in thin sectioned virus. The resultant images from both negative staining and thin sectioning suggest that the organization of the core is unique amongst intracytoplasmic deoxyriboviruses (ICDVs).