Germline Transgenic Methods for Tracking Cells and Testing Gene Function during Regeneration in the Axolotl

(Stem Cell Reports 1, 90–103; June 4, 2013) The following was omitted from Acknowledgments section of the original article: Elly Tanaka was also funded by the ERC Advanced Investigator Award. Germline Transgenic Methods for Tracking Cells and Testing Gene Function during Regeneration in the AxolotlKhattak et al.Stem Cell ReportsJune 04, 2013In BriefThe salamander is the only tetrapod that regenerates complex body structures throughout life. Deciphering the underlying molecular processes of regeneration is fundamental for regenerative medicine and developmental biology, but the model organism had limited tools for molecular analysis. We describe a comprehensive set of germline transgenic strains in the laboratory-bred salamander Ambystoma mexicanum (axolotl) that open up the cellular and molecular genetic dissection of regeneration. We demonstrate tissue-dependent control of gene expression in nerve, Schwann cells, oligodendrocytes, muscle, epidermis, and cartilage.