The Coumarin Glycosides of Daphne acuminata: Use of13C–NMR Spectroscopy for their Identification

Two coumarin glycosides previously isolated from Daphne acuminata Royle have been shown to be daphnin and daphnetin–8–β–glucoside. The value of 13 C–NMR spcectroscopy for the identification of coumarin glycosides is demonstrated. Its use in characterising the glycosidic moiety and confirming the 7, 8– coumarin substitution pattern of daphnetin–8–β–glucoside, a previously reported constituent of D. acuminata, is shown. Key Word Index Daphne acuminata - Coumarin Glycosides - Daphnin - Daphnetin–8–β–glucoside