A description is given of the construction and operation of a system of chambers in which plants can be grown in filtered air, with or without the addition of SO2. Concentrations of SO2 as low as 20 μg m−3, which can be maintained in the chambers over prolonged periods, are monitored automatically by conductirnetric and colorirnetric methods of analysis. The plants are grown in pots containing soil and are watered by remote control, allowing exposure to SO2 for extended periods without interruption. The method of watering is based on loss of weight and gives an estimate of transpiration. A brief account is given of an experiment in which perennial ryegrass (Lolium perenne L.) cv. S23 was grown in the chambers and exposed to filtered air containing 0, 50, 100, 200, and 400 g μg m−3 SO2. Exposure to low concentrations of SO2 alleviated sulphur deficiency but at the highest concentration there was evidence of a reduction in yield without visible signs of injury.
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Lockyer et al. (1976) studied this question.