Dietary Choline and the Maintenance of the Cardiovascular System in Rats

0.05 g.; Al1(SO4)3.K2SO4.24H2O, 0.40 g.; CoCI5.6H, 005 g.; Na.SiOs.9H2O, 2 g.; and NaAsO2, 0.10 g.t The composition of vitamin mixture (Canad.J. med.Sci., 1953, 31, 135) was as follows: thiamine hydrochloride, 500 mg.; riboflavine, 250 mg.; pyridoxine hydrochloride, 200 mg.; calcium pantothenate, I g.; nicotinic acid, I g.; folic acid, 50 mg.;biotin, 30 mg.; 2-methyl-1,4-naphthoquinone, 100 mg.; p-aminobenzoic acid, 10 g.; inositol, 50 g.; and finely powdered sucrose (100 mesh) to 1,000 g. t Cod-liver oil concentrate (0 01%/) and alpha-tocopherol acetate (0.015°') supplemented all diets but that used in the preliminary experiment (Expt.A).Obtained from Ayerst, McKenna, and Harrison, Ltd., Montreal; contains 200,000 I.U.vitamin A and 50,000 I.U.vitamin D per g.laurate.This diet was semi-liquid, and the food intake of rats maintained on it was low.Diet B was therefore devised to secure a more solid state by the inclusion of a large amount (15%0) of cellu-flour.The food intake of animals