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September 18, 2026Biochemistry and Biophysics ReportsOpen Access

Lipidomic profiling of PEX11β knockout T-REx 293 cells reveals lipid shifts suggesting potential metabolic perturbations

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Population

Human embryonic kidney-derived cell line (T-REx 293 cells) with peroxin knockouts (PEX3, PEX14, PEX11β)

Comparison

Peroxin knockouts (PEX3, PEX14, PEX11β) vs Wild-type T-REx 293 cells

Design

Preclinical

Key result

PEX11β knockout cells showed a lipid profile similar to wild-type except for highly enriched ceramides, contrasting with PEX3 and PEX14 knockouts which were enriched in very-long-chain fatty acids.

Authors

SJSamantha JengMSMansha SoniVKVishal C. Kalel

Discussion

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Overview

These peroxin KO profiles should not change practice; hypothesis-generating for peroxisomal redox targeting in metabolic disorders.

Key Points

  • To characterize and compare the lipidomic fingerprints and metabolic consequences of knocking out specific peroxin genes in human cell models.
  • Conducted untargeted lipidomic profiling on human embryonic kidney-derived T-REx 293 cells across wild-type and peroxin knockout (PEX3, PEX14, and PEX11β KO) backgrounds.
  • Assessed cellular redox vulnerability across cell lines following pharmacological inhibition of glucose-6-phosphate dehydrogenase (G6PD).
  • PEX3 and PEX14 KO cells displayed massive enrichment of very-long-chain fatty acids and depletion of ether- and plasmalogen-linked phospholipids, with PEX3 KO driving stronger deviations from wild-type profiles.
  • PEX11β KO cells closely mirrored wild-type lipid profiles overall, but exhibited selective and prominent enrichment of specific lipids, particularly ceramides.
  • The peroxin knockout cell lines exhibited divergent survival responses to G6PD inhibition, reflecting distinct redox stress dynamics and reliance on cellular NADPH.

Structured PICO

P
Population
Human embryonic kidney-derived cell line (T-REx 293 cells) with peroxin knockouts (PEX3, PEX14, PEX11β)
I
Intervention
Peroxin knockouts (PEX3, PEX14, PEX11β)
C
Comparator
Wild-type T-REx 293 cells
O
Outcome
Lipidomic profile changes and response to glucose-6-phosphate dehydrogenase (G6PD) inhibitionsurrogate

Different peroxin knockouts in human embryonic kidney cells produce distinct lipidomic profiles and metabolic adaptations, highlighting specific roles in lipid metabolism and redox stress.

Cite This Study

Jeng et al. (2026) studied Peroxisome biogenesis disorders. Peroxin knockouts (PEX3, PEX14, PEX11β) vs. Wild-type cells was evaluated on Lipidomic profile and response to G6PD inhibition. PEX11β knockout cells showed a lipid profile similar to wild-type except for highly enriched ceramides, contrasting with PEX3 and PEX14 knockouts which were enriched in very-long-chain fatty acids.

synapsesocial.com/papers/6aacf5ed0c46fbdff987dd5fhttps://doi.org/10.1016/j.bbrep.2026.102801
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