Key result
PCR detects ALV subgroup E in sick chickens linked to significant cardiac and multi-organ lesions.
Why the study?
Efficient and accurate detection and prevention of avian leukemia virus in chickens are needed to reduce economic losses in the global chicken industry.
Case-Control (n=60)
No
PCR is a simple, direct, and highly accurate method for diagnosing suspected cases of viral leukemia infection in farm chickens.
Associates ALV-E with cardiac lesions in chickens; hypothesis-generating for retroviral models, leaves open human translation.
Chicken leukemia caused by avian leukemia virus has been troubling the global chicken industry, and its efficient and accurate detection and prevention can effectively reduce economic losses.This article aims to conduct pathogen analysis and identify a batch of suspected cases of chicken leukemia in a free-range chicken farm in Zhangshu City, Jiangxi Province, providing a basis for the disease diagnosis and treatment.Suspected chicken leukemia in broiler chickens was screened through clinical, pathological observations and H&E staining, then confirmed by PCR.According to clinical and anatomical diagnosis, the diseased chicken was weaker and unable to stand than the healthier broiler chicken of the same age.H&E staining showed heart, liver, spleen, lungs, and kidney lesions.The PCR and electrophoresis analysis detected a specific band of 405bp, which was consistent with the size of the target gene fragment.Therefore, these suspected cases were diagnosed as avian leukemia virus infection.The results indicated that the PCR is simple, direct, and highly accurate and can diagnose suspected cases of viral leukemia infection in farm chickens.
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A 2024 study conducted a case-control in Avian Leukemia Virus (ALV) infection (n=60). Avian Leukemia Virus (ALV) infection vs. Healthy chickens was evaluated on Detection of ALV by PCR and histopathological changes. PCR successfully detected a 405bp specific band for ALV subgroup E in sick chickens, which also exhibited significant histopathological lesions in the heart, liver, spleen, lungs, and kidneys compared to healthy chickens.
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