Effects of Abies sibirica terpenes on cancer- and aging-associated pathways in human cells

// Anna Kudryavtseva 1, 2, * , George Krasnov 1, * , Anastasiya Lipatova 1 , Boris Alekseev 2 , Faniya Maganova 3 , Mikhail Shaposhnikov 4 , Maria Fedorova 1 , Anastasiya Snezhkina 1 , Alexey Moskalev 1, 4, 5 1 Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia 2 National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Moscow, 125284, Russia 3 Initium-Pharm, LTD, Moscow, 142000, Russia 4 Institute of Biology of Komi Science Center of Ural Branch of RAS, Syktyvkar, 167982, Russia 5 Moscow Institute of Physics and Technology, Dolgoprudny, 141700, Russia * These authors have contributed equally to this work Correspondence to: Alexey Moskalev, email: amoskalev@ib.komisc.ru Keywords: Abies sibirica, plant extract, terpenoids, anti-aging effects, anti-cancer effects Received: August 12, 2016 Accepted: October 19, 2016 Published: November 19, 2016 ABSTRACT A large number of terpenoids exhibit potential geroprotector and anti-cancer properties. Here, we studied whole transcriptomic effects of Abisil, the extract of fir ( Abies sibirica ) terpenes, on normal and cancer cell lines. We used early passaged and senescent none-immortalized fibroblasts as cellular aging models. It was revealed that in normal fibroblasts, terpenes induced genes of stress response, apoptosis regulation and tissue regeneration. The restoration of the expression level of some prolongevity genes after fir extract treatment was shown in old cells. In Caco-2 and AsPC-1 cancer cell lines, Abisil induced expression of both onco-suppressors (members of GADD45, DUSP, and DDIT gene families), and proto-oncogenes ( c-Myc , c-Jun , EGR and others). Thus, the study demonstrates the potential anti-aging and anti-cancer effects of Abisil on senescent and cancer cell lines.