What question did this study set out to answer?

To establish a restriction fragment length polymorphism (RFLP) method using nuclear DNA extracted from grapevine wood to identify rootstocks and differentiate clonal lines.

October 9, 2015Open Access

Identification of sixteen grapevine rootstocks by RFLP and RFLP analysis of nuclear DNA extracted from the wood

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Authors

JBJoël BourquinUniversity of Fribourg PTP. Tournier LOLéon OttenInstitut de Biologie Moléculaire des Plantes

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Implication

Experimental study reveals reliable identification of sixteen grapevine rootstocks in Vitis cultivars, indicating an effective molecular alternative to traditional ampelography.

Key Points

To establish a restriction fragment length polymorphism (RFLP) method using nuclear DNA extracted from grapevine wood to identify rootstocks and differentiate clonal lines.
Extracted nuclear DNA from woody tissues and leaves across 16 grapevine rootstocks within the Vitis genus.
Digested DNA samples with the HinfI restriction endonuclease and hybridized them using low-copy nuclear DNA probes from Vitis vinifera cv. Chardonnay.
Analyzed clonal polymorphism across 5 clones of SO 4 (V. berlandieri x V. riparia) and 3 clones of 41 B Mgt (V. berlandieri x V. vinifera) using 4 probes.
Successfully identified and distinguished all 16 Vitis rootstocks using HinfI digestion and nuclear DNA probes.
Obtained identical RFLP hybridization patterns between DNA extracted from wood and DNA extracted from leaves.
Detected no intra-cultivar polymorphism among the 5 clones of SO 4 or the 3 clones of 41 B Mgt.

Cite This Study

Bourquin et al. (2015) studied this question.

synapsesocial.com/papers/6aad0533555e610345f4905e https://doi.org/10.5073/vitis.1992.31.157-162

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Implication

Experimental study reveals reliable identification of sixteen grapevine rootstocks in Vitis cultivars, indicating an effective molecular alternative to traditional ampelography.

Identification of sixteen grapevine rootstocks by RFLP and RFLP analysis of nuclear DNA extracted from the wood

Discussion

Member takes

Implication

Key Points

Cite This Study

Identification of sixteen grapevine rootstocks by RFLP and RFLP analysis of nuclear DNA extracted from the wood

Discussion

Member takes

Implication

Key Points

Cite This Study

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