Key result
In situ hybridization and immunohistochemistry successfully detect CSFV in tissues of chronically infected pigs.
Why the study?
Detection and localization of classical swine fever virus nucleic acid and antigen in chronically infected pigs was needed to study the pathogenesis of chronic CSFV infection.
Observational (n=15)
In situ hybridization is a useful technique for detecting CSFV RNA in tissues from chronically infected pigs, aiding in pathogenesis studies.
Aids chronic CSFV pathogenesis research in swine; leaves open translation to clinical diagnostics.
Classical swine fever (CSF) virus (CSFV) nucleic acid and antigen were detected in 15 pigs with naturally occurring chronic CSF by in situ hybridization and immunohistochemistry. The most consistent and prominent microscopic lesions were perivascular mononuclear cell infiltration and gliosis in the central nervous system of pigs with chronic CSF. Positive cells typically exhibited a dark brown (in situ hybridization) or red (immunohistochemistry) reaction product in the cytoplasm without background staining. A positive signal for both in situ hybridization and immunohistochemistry was detected in mononuclear cells and lymphocytes of lymphoid tissues. Viral nucleic acid was detected in some tissue sections in the absence of viral antigen. The in situ hybridization technique developed in this study was useful for the detection of CSFV RNA in tissues taken from chronically infected pigs and may be a valuable technique for studying the pathogenesis of chronic CSFV infection.
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Choi et al. (2003) conducted an observational in Naturally occurring chronic classical swine fever (CSF) (n=15). In situ hybridization and immunohistochemistry was evaluated on Detection of CSFV nucleic acid and antigen. In situ hybridization and immunohistochemistry successfully detected classical swine fever virus nucleic acid and antigen in tissues from 15 chronically infected pigs.
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