What question did this study set out to answer?

Investigate how circular RNAs associate with cellular proteins in mammalian cells and identify specific circRNP subfamilies defined by the RNA-binding protein IMP3.

August 11, 2016Scientific ReportsOpen Access

CircRNA-protein complexes: IMP3 protein component defines subfamily of circRNPs

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Authors

TSTim SchneiderJustus-Liebig-Universität Gießen LHLee-Hsueh HungJustus-Liebig-Universität Gießen SSSilke SchreinerJustus-Liebig-Universität Gießen

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Implication

Biochemical analysis reveals distinct circular RNA-protein complexes defined by the RNA-binding protein IMP3 in mammalian cells, suggesting the existence of specialized circRNP families.

Key Points

Investigate how circular RNAs associate with cellular proteins in mammalian cells and identify specific circRNP subfamilies defined by the RNA-binding protein IMP3.
Characterized circRNP complex sizes and translation efficiency in HeLa cell lysates using glycerol gradient centrifugation and polysome-gradient fractionation.
Identified and validated IMP3-bound circular RNAs by combining RNA sequencing of IMP3-co-immunoprecipitated RNA with bioinformatic filtering for circular-junction reads.
Glycerol and polysome gradient analyses showed that circRNPs form complexes of distinct sizes with no evidence of efficient translation among abundant circular RNAs in HeLa cells.
RNA sequencing of IMP3 immunoprecipitates isolated a distinct set of circular RNAs, confirming that specific circRNP subfamilies are defined by a shared RNA-binding protein partner.

Cite This Study

Schneider et al. (2016) studied this question.

synapsesocial.com/papers/6aad0909c9e34414aa3de995 https://doi.org/10.1038/srep31313

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Implication

Biochemical analysis reveals distinct circular RNA-protein complexes defined by the RNA-binding protein IMP3 in mammalian cells, suggesting the existence of specialized circRNP families.

CircRNA-protein complexes: IMP3 protein component defines subfamily of circRNPs

Discussion

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Implication

Key Points

Cite This Study

CircRNA-protein complexes: IMP3 protein component defines subfamily of circRNPs

Discussion

Member takes

Implication

Key Points

Cite This Study

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