// Shuang Liang 1 , Jing Guo 1 , Jeong-Woo Choi 1 , Kyung-Tae Shin 1 , Hai-Yang Wang 1 , Yu-Jin Jo 1 , Nam-Hyung Kim 1 , Xiang-Shun Cui 1 1 Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, 361–763, Republic of Korea Correspondence to: Xiang-Shun Cui, email: xscui@cbnu.ac.kr Nam-Hyung Kim, email: nhkim@cbun.ac.kr Keywords: PP2A-B55α, oocyte maturation, cytokinesis, preimplantation development, reproduction Received: December 08, 2016 Accepted: February 17, 2017 Published: March 06, 2017 ABSTRACT Protein phosphatase 2A regulatory subunit B55α (PP2A-B55α) has been studied in mitosis. However, its functions in mammalian meiosis and early embryonic development remain unknown. Here, we report that PP2A-B55α is critical for mouse oocyte meiosis and preimplantation embryo development. Knockdown of PP2A-B55α in oocytes led to abnormal asymmetric division, disordered spindle dynamics, defects in chromosome congression, an increase in aneuploidy, and induction of the DNA damage response. Moreover, knockdown of PP2A-B55α in fertilized mouse zygotes impaired development to the blastocyst stage. The impairment of embryonic development might have been due to induction of sustained DNA damage in embryos, which caused apoptosis and inhibited cell proliferation and outgrowth potential at the blastocyst stage. Overall, these results provide a novel insight into the role of PP2A-B55α as a novel meiotic and embryonic competence factor at the onset of life.
No takes yet. Share an insight, caveat, or question.
Liang et al. (2017) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: