Key result
Elevated PON2 in bladder cancer is linked to increased tumor proliferation and oxidative stress resistance.
Why the study?
The role of paraoxonase-2 (PON2) in bladder cancer and its potential as a biomarker and prognostic factor was not well understood.
Observational
PON2 is overexpressed in bladder cancer and promotes tumor cell proliferation and resistance to oxidative stress, suggesting its potential as a biomarker and prognostic factor.
Hypothesis-generating for PON2 targeting in bladder cancer; leaves open translation to human outcomes.
// Tiziana Bacchetti 1, * , Davide Sartini 2, * , Valentina Pozzi 3 , Tiziana Cacciamani 1 , Gianna Ferretti 2 , Monica Emanuelli 2, 3 1 Department of Life and Environmental Sciences, Polytechnic University of Marche, Ancona, Italy 2 Department of Clinical Sciences Polytechnic University of Marche, Ancona, Italy 3 New York-Marche Structural Biology Center (NY-MaSBiC), Polytechnic University of Marche, Ancona, Italy * These authors have contributed equally to this work Correspondence to: Gianna Ferretti, email: g.ferretti@univpm.it Keywords: paraoxonase-2, oxidative stress, bladder cancer, biomarker, antioxidant Received: October 24, 2016 Accepted: February 06, 2017 Published: February 24, 2017 ABSTRACT The enzyme paraoxonase-2 (PON2) is ubiquitously expressed and exerts its antiapoptotic and antioxidative functions in several intracellular compartments. The aim of this study is to investigate the role of PON2 in bladder cancer (BC). The expression levels of PON2 in paired tumor and normal bladder tissue samples and in urinary exfoliated cells from patients affected with BC and healthy donors were evaluated. Moreover, the effect of PON2 overexpression on tumor cell proliferation and susceptibility to oxidative stress was investigated in human bladder cancer cell line T24. Our results showed that PON2 expression levels were significantly higher in BC compared with non-tumor tissue. In urinary exfoliated cells from BC patients, PON2 mRNA levels showed an inverse correlation with tumor stage (pT). Moreover, PON2 overexpression in T24 cells led to a significant increase in tumor cell proliferation and resistance to oxidative stress. The results obtained showed that PON2 could represent a molecular biomarker for bladder cancer and suggest a potential role of the enzyme as a prognostic factor for this neoplasm.
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Bacchetti et al. (2017) conducted an observational in Bladder cancer. PON2 expression and overexpression vs. Normal bladder tissue, healthy donors, and non-overexpressing cells was evaluated on PON2 expression levels and effect on tumor cell proliferation and oxidative stress. PON2 expression was significantly higher in bladder cancer tissue compared to normal tissue, and its overexpression increased tumor cell proliferation and resistance to oxidative stress.
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