Key result
Two newly developed porcine macrophage cell lines exhibit characteristic morphology and phagocytic function.
Why the study?
Porcine macrophage biology requires useful in vitro tools, but characterized porcine monocyte-derived macrophage cell lines were lacking.
The newly developed porcine monocyte-derived macrophage cell lines CΔ2+ and CΔ2− exhibit typical macrophage characteristics and will serve as useful tools for studying porcine macrophage biology.
May aid preclinical porcine immunology models; leaves open validation for broader research use.
Cell lines CΔ2+ and CΔ2− were developed from monocytes obtained from a 10-month-old, crossbred, female pig. These cells morphologically resembled macrophages, stained positively for α-naphthyl esterase and negatively for peroxidase. The cell lines were bactericidal and highly phagocytic. Both cell lines expressed the porcine cell-surface molecules MHCI, CD11b, CD14, CD16, CD172, and small amounts of CD2; however, only minimal amounts of CD163 were measured. The lines were negative for the mouse marker H2Kk, bovine CD2 control, and secondary antibody control. Additionally, cells tested negative for Bovine Viral Diarrhea Virus and Porcine Circovirus Type 2. Therefore, these cells resembled porcine macrophages based on morphology, cell-surface marker phenotype, and function and will be useful tools for studying porcine macrophage biology.
No takes yet. Share an insight, caveat, or question.
Chitko-McKown et al. (2013) studied this question. Development of porcine monocyte-derived macrophage cell lines CΔ2+ and CΔ2− was evaluated on Cell line characterization (morphology, surface markers, function). Two newly developed porcine monocyte-derived macrophage cell lines, CΔ2+ and CΔ2−, exhibited macrophage-like morphology, phagocytic function, and characteristic cell-surface markers.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: