Key result
GST-tagged PIN unexpectedly inhibits all NOS isoenzymes with an IC50 of ~18 microM.
Why the study?
The specific interaction and inhibitory effects of the protein inhibitor of neuronal nitric oxide synthase (PIN) on pure NOS enzymes were not fully characterized.
Effect estimate: IC50 18 +/- 6 microM
GST-tagged PIN inhibits all NOS isoenzymes, not just nNOS, prompting a reconsideration of its specific physiological role.
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GST-PIN inhibits all NOS isoforms in vitro; challenges nNOS specificity and leaves open physiological relevance.
Hemmens et al. (1998) studied this question. GST-tagged PIN was evaluated on Inhibition of NOS isoenzymes (IC50 18 +/- 6 microM). GST-tagged PIN unexpectedly inhibited all NOS isoenzymes, with an IC50 for nNOS of 18 +/- 6 microM, while leaving cytochrome c reductase activity and dimerization unaffected.
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