An improved method is described for the extraction of soluble proteins from intact and in-vitro-cultured plant tissues. Its main characteristics are the elimination of contaminants, concentration and stability of extracts, and suitability for acrylamide gel electrophoresis. The usefulness of the method was demonstrated by depicting the differences in protein complements of intact cotyledons, callus tissue, and cell suspension cultures of flax.
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Ibrahim et al. (1975) studied this question.
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