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Sialic acid isolated from submaxillary gland and also from casein, and synthetic sialic acid have been used as standards in the determination of sialic acid in casein by the thio‐barbituric acid method. Consistently higher results for the apparent sialic acid content of casein were obtained with the synthetic sialic acid than with the sialic acid obtained from biological materials. A study has been made of the effects of conditions of hydrolysis on the apparent concentration of sialic acid in whole casein.
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Huang et al. (1964) studied this question.
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