Key result
Pulsed direct current electric fields alter epileptiform burst intervals in rat hippocampal slices.
Why the study?
The effects of pulsed direct current electric fields on spontaneous bursting frequency in a high potassium hippocampal slice model of epileptic focus were studied to understand modulation of burst firing activity.
Do pulsed direct current electric fields alter the frequency of spontaneous bursting in a rat hippocampal slice model of epileptic focus?
Do pulsed direct current electric fields alter the frequency of spontaneous bursting in a rat hippocampal slice model of epileptic focus?
Pulsed direct current electric fields can significantly alter epileptiform burst firing activity in a high potassium rat hippocampal slice model.
Alters burst frequency in high-K+ hippocampal slices at specific strengths; leaves open translation to in vivo epilepsy models or neuromodulation.
The effects of pulsed direct current (dc) electric fields on the frequency of spontaneous bursting in a model epileptic focus were studied. The high potassium hippocampal slice model was used to generate spontaneous burst firing activity similar to interictal spikes in the pyramidal cell layer of CA3. Electric fields were generated from platinum subdural electrodes placed in the perfusion bath. Three hundred and seventy-eight experimental trials were performed on 10 hippocampal slices from 10 rats and the effects of field polarity, field strength and duration of stimuli on firing frequency was examined. Hippocampal slices were oriented horizontally with the CA3 layer towards the positive electrode, the average interburst interval did not correlate significantly with polarity of the delivering pulses (one-way ANOVA, p = 0.96). Average interburst interval showed a significant correlation with pulse duration of 200 and 400 msec (p = 0.030 and p = 0.004, respectively). As a function of field strength, there were significant average interval changes for fields of 33, 46, and 73 mV/mm (p = 0.024, p = 0.001 and p = 0.001, respectively). In conclusion, CA3 burst firing activity in high potassium concentration can therefore be altered by electric fields.
No takes yet. Share an insight, caveat, or question.
Duong et al. (1998) studied Epileptiform bursts (model epileptic focus) (n=10). Pulsed direct current (dc) electric fields was evaluated on Average interburst interval (frequency of spontaneous bursting) (p=0.001). Pulsed direct current electric fields significantly altered the average interburst interval of epileptiform bursts in rat hippocampal slices depending on pulse duration and field strength (P=0.001).
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: