Key result
Adenovirus-mediated c-myb ribozyme inhibits SMC proliferation and neointima formation in rat injury models.
Why the study?
Smooth muscle cell proliferation contributes to restenosis after balloon angioplasty, and inhibition of proliferation can limit neointima hyperplasia in animal models.
Does recombinant adenovirus expressing ribozymes against c-myb mRNA prevent neointima formation in a rat carotid artery balloon injury model?
Does recombinant adenovirus expressing ribozymes against c-myb mRNA prevent neointima formation in a rat carotid artery balloon injury model?
Adenovirus-mediated delivery of a ribozyme against c-myb mRNA effectively reduces smooth muscle cell proliferation and neointima formation in a preclinical model of restenosis.
Answers are grounded in this paper and cite their sources.
Does not support clinical use; leaves open human translation of c-myb ribozymes for restenosis.
Smooth muscle cell (SMC) proliferation is an important component of restenosis in response to injury after balloon angioplasty. Inhibition of proliferation in vivo can limit neointima hyperplasia in animal models of restenosis. Ribozymes against c-myb mRNA have been shown to be effective inhibitors of SMC proliferation in vitro. The effectiveness of adenovirus as a gene therapy vector in animal models of restenosis is well documented. In order to test the utility of ribozymes to inhibit SMC proliferation by a gene therapy approach, recombinant adenovirus expressing ribozymes against c-myb mRNA was generated and tested both in vitro and in vivo. This adenovirus ribozyme vector is shown to inhibit SMC proliferation in culture and neointima formation in a rat carotid artery balloon injury model of restenosis.
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Macejak et al. (1999) studied Restenosis. Recombinant adenovirus expressing ribozymes against c-myb mRNA was evaluated on Smooth muscle cell proliferation and neointima formation. Adenovirus-mediated expression of a ribozyme against c-myb mRNA inhibited smooth muscle cell proliferation in culture and neointima formation in a rat carotid artery balloon injury model.
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